Fall 2016 |
Complete readings BEFORE class using (syllabus pdf ) |
Read using Nota Bene |
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Monday |
Week 1 : Course introduction (slides) An introduction to why model systems and why Xenopus. |
readings & |
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| Monday 26 Aug lab A250 |
An introduction to online resources: PubMed, OMIM, and Xenbase. A short presentation on genes of choice Lab Safety review, Good Lab Practices, Embryo injections |
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| Wed. 28 Aug lab A250 |
Ethical use of animals in research What makes a gene interesing? |
NB: Portin & Wilkins. 2017. The Evolving Definition of the Term “Gene” Notes on CRISPR Notes on laboratory saftey |
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Monday |
Week 2 Labor day - no class |
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| Weds. 4 Sept. |
A further introduction to the lab: what controls are relevant. Pipetting techniques, Molarity calculations |
Reading on Xenopus |
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Monday |
Week 3 |
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Monday |
Gene mutagenesis with sgRNA+Cas9 Inject sgRNA and Cas9 mRNA into Xenopus embryos (tyrosinase control +) |
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Wed. |
Analysis of injected embryos Fixation and preparation for analysis |
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| Monday 16 Sept. lecture |
Week 4 |
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| Monday 16 Sept. lab |
Generation of template DNA for in situ hybridization probes | ||||
| Weds. 18 Sept. lab. |
Confirm template and probe synthesis |
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Monday |
Week 5 Short presentations on gene of choice |
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| Monday 23 Sept lab |
Building the sgRNA template plasmids |
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Weds. |
Building the sgRNA template plasmids | ||||
| Monday 30 Sept lecture |
Week 6 |
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| Monday 30 Sept lab |
Confirmation of sgRNA template plasmid |
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| Weds. 2 Oct. lab |
Confirmation of sgRNA template plasmid |
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Monday |
Week 7 Modeling human development and disease in Xenopus |
science literacy survey return wednesday |
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| Monday 7 Oct. lab |
First embryo injection | ||||
| Weds 9 Oct. lab |
First embryo injection |
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| Monday 14 Oct. llecture |
Week 8 How to analyze data and generate figures (introduction to statistics) |
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| Monday 14 Oct. lab |
Injection of Xenopus embryos with gRNA constructs |
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| Weds 16 Oct. lab |
continue |
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Monday |
Week 9 How to analyze data and generate figures (continue) |
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| Monday 21 Oct lab |
Phenotype analysis and Sample preparation for genotyping (continued) |
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| Weds 23 Oct lecture |
` | continue |
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| Monday 28 Oct lecture |
Week 10 Start storyboards for CURE symposium |
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| lab | Continue with injection studies and analysis |
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| Weds 30 Oct lab |
Continue with injection studies and analysis |
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| Monday 4 Nov lecture |
Week 11 |
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| Monday 4 Nov lab |
Analysis and evaluation of results and troubleshooting II (Phenotypes and Genotypes) |
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| Weds 6 Nov lab |
continue | ||||
| Monday 11 Nov lecture |
Week 12 |
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| lab | Analysis and evaluation of results and troubleshooting II (Phenotypes and Genotypes) |
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| Weds 13 Nov lab |
continue | ||||
| Monday 18 Nov lecture |
Week 13 Discussion of results / new experiments? |
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| lab | Analysis and evaluation of results and troubleshooting II (Phenotypes and Genotypes) |
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| Weds 20 Nov lab |
continue | ||||
| 21-29 Nov. | Thanksgiving break |
be thankful |
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| Monday 2 Dec lecture |
Week 14 present storyboards for final presentations |
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| lab | storyboards (and clean up studies) |
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| Weds 4 Dec lab |
storyboards (and clean up studies) |
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| Monday 9 Dec. lecture |
Week 15 |
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| lab | Presentations of talks / storyboards | ||||
| Weds 11 Dec. lab |
Presentations of talks / storyboards | ||||
| 16 December 2015 4:30 PM - 7:00 PM |