Prerequisites: MCDB 3135 & 3145 or by instructor consent for students from other majors.

Assigned readings through the nota bene system
pre-class formative assessment using
beSocratic@CU Boulder

CRISPR-Cas9 genome editing with applications for treating genetic diseases. Further information at the Zhang lab website or in this iBiology video by Jennifer Doudna


Overview: A three credit discovery laboratory course to examine the role of genes (that you select) during early X. laevis development using CRISPR-Cas9 and variations of it.

You will use on-line databases to select a gene of interest, design and build the reagents needed to generate and confirm the presence of mutations or the suppression of its expression, and characterize the resulting phenotypes (if any).

You will discuss with and present your findings to your peers and the world through storyboards, posters, and talks.

Lecture: Monday, 10AM-10:50AM in  Gold A350
Lab hours: Monday + Wednesday, 11AM to 12:50PM         
Location: Porter E0040

Instructors: Bilge Birsoy, Ph.D. Office hours: TBD
Mike Klymkowsky Office hours: Weds 3-5
425 Porter or by appointment.

Synchronous development of X. laevis embryos. Gastrulation follows the cleavage stage. Williams & Smith. – for more go to xenbase.org