Fall 2016

   

Complete readings BEFORE class using (syllabus pdf )

 

Read using Nota Bene





 
           
           
Monday
22 Aug.
   

Week 1 Introduction to the Scientific Process & Model Organisms,
Genetics and Molecular Biology review

Scientific Process, Model Organisms (including Xenopus laevis) & embryology

 

readings &
↓ assignments ↓
genetics survey

           
Tuesday
23 Aug.
    Introduction to Genotype and Phenotype, What makes a gene interesing?
 

introductory slides
NB reading for Thursday: ExAC

Thursday
25 Aug.
   

Introduction to PubMed, Xenbase, (and Nota Bene)

 

session slides





 
Monday
29 Aug.
   

Week 2:Introduction to Experimental Design and Molecular Biology Techniques, Online databases, CRISPR-Cas9 and Oligo Design

Gene expression and mutagenesis, (promoters, transcription, types of mutations and their effect on mRNA and protein synthesis)

 
John Gurdon's
Nobel video | slides

session slides
           
Tuesday
30 Aug
   


Online database use, Experimental Design, MolBio Techniques

 

Designing your gRNA construct
pdf | word

Thursday
1 Sept
    Short presentations on gene of choice  

science literacy survey
return tuesday




 
Monday
5 Sept
   


NO CLASS - Labor Day Holiday

   
           
Tuesday
6 Sept
   

Week 3: Laboratory Safety Training and Design and Cloning of gRNA and in situ probe constructs

CRISPR sgRNA constructs, oligo design

 

Notes on CRISPR
Notes on laboratory saftey

Thursday
8 Sept
    Lab Safety Training, Good Lab Practices, Pipetting techniques, Molarity calculations
 
Ordering oligos (moving into the lab)




 
Monday
12 Sept
    Week 4:lecture (TBA)
   
           
Tuesday
13 Sept
   
 
           
Thursday
15 Sept
   
   




 
Monday
19 Sept
    Week 5:lecture (TB)    
           
Tuesday
20 Sept
    Analysis and evaluation results and troubleshooting I (Cloning)
Analyze results, troubleshoot and repeat the necessary steps
   
Thursday
22 Sept
    continue
   

   
 
Monday
26 Sept
    Week 6: (TBA)
 

 

           
Tuesday
27 Sept
   

Injection of Xenopus embryos with gRNA constructs
Embryo injections, Phenotype analysis and sample preparation (freeze and fix samples regardless of phenotype)

 

 

Thursday
29 Sept
    continue
 

 





   
Monday
3 Oct
    Week 7:lecture (TBA)
   
           
Tuesday
4 Oct.
    Phenotype analysis and Sample preparation for genotyping (continued)
 

 

Thursday
6 Oct.
  ` continue
   




 
Monday
10 Oct.
    Week 8:lecture (TBA)    
           
Tuesday
11 Oct.
    Genomic PCR and Genotyping: PCR uninjected controls along with gRNA injected samples Both for tyrosinase control as well as genes of interest
   
Thursday
13 Oct.
    continue
   




 
Monday
17  Oct.
    Week 9:lecture (TBA)
 
           
Tuesday
18 Oct.
    Analysis and evaluation of results and troubleshooting II (Phenotypes and Genotypes)
 
Thursday
20 Oct.
    continue    




 
Monday
24  Oct.
    Week 10:lecture (TBA)
 
Tuesday
25 Oct.
   

In situ hybridization; Probe synthesis, dot blots, start in situs if probes are good

   
Thursday
27 Oct.
    continue
   




 
Monday
31 Oct.
    Week 11:lecture (TBA)
 

 

           
Tuesday
1 Nov.
   

In situ hybridization continued

 

 

Thursday
3 Nov.
    continue
 




 

Monday
7 Nov.

   

Week 12:lecture (TBA)

   
           

Tuesday
10 Nov.

    Analysis and evaluation of results and troubleshooting III (In situ hybridization)    
Thursday
12 Nov.
    continue
 

   
 
Monday
14 Nov.
    Week 13:lecture (TBA)
   
           
Tuesday
15 Nov.
    How to generate figures and present data
 


Thursday
17 Nov.
    continue
 

 


   
 
21-25 Nov.    
Thanksgiving break
 
be thankful




 
Monday
28 Nov.
    Week 14: present storyboards for final presentations
 

 

           
Tuesday
29 Nov.
    storyboards (and clean up studies)
 


Thursday
1 Dec.
    storyboards (and clean up studies)
   




 

Monday
5 Dec.

    Week 15:lecture (TBA)    
           
Tuesday
6 Dec.
    group presentations (movie time)    
Thursday
8 Dec.
    group presentations (movie time)    




 
          16 December 2015 4:30 PM - 7:00 PM