Prerequisites: MCDB 3135 & 3145 or by instructor consent for students from other majors.

Assigned readings through the nota bene system
pre-class formative assessment using
beSocratic@CU Boulder


Overview: A three credit discovery laboratory course to examine the role of genes (that you select) during early X. laevis development using CRISPR-Cas9. You will use on-line databases to select a gene of interest (not previously studied in Xenopus), formulate hypotheses about the gene's function, design and build the reagents needed to generate and confirm the presence of mutations in that gene, and characterize the mutant phenotype in terms of gene expression and embryonic phenotype. You will discuss with and present your findings to your peers and the world through a short video presentation.

Lecture hour: Monday, 10AM-10:50AM in  Porter B436

Lab hours: Tuesday-Thursday, 2PM to 4PM         
Location: Porter B425

Instructors: Bilge Birsoy, Ph.D. Office hours: TBD
Mike Klymkowsky : website Office hours: TBD

Synchronous early development of X. laevis embryos. Gastrulation follows the cleavage stage; migrating and involuting mesoderm underlies the prospective ectoderm.
Williams & Smith. For more go to xenbase.org
CRISPR-Cas9 method of genome editing &ndash with many applications including the potential to treat genetic diseases. Further information can be found at the Zhang lab website or in this iBiology video by Jennifer Doudna