title graphic

DEVO@CU is an attempt to build a coherent introduction to the key ideas, observations, and model systems used in the study of developmental biology. 

DEVO is part of a larger curricular redesign effort that includes biofundamentals, CLUE: Chemistry, life, the universe & everything & Organic CLUE (O!CLUE)(testimonial).   DEVO uses nota bene interactive reading and beSocratic activities (due before class) that are the focus of in class activities and discussion.  

Videos on using the text & constructing arguments.

 

overview

 
 
Syllabus
Date
Topics
- working
 
complete before class:
readings, questions & activities  working


 

Tuesday
15 January

Introduction

keynote pdf

 

 

 

Introduction to the course: rules, expectations & such
Introduction to Nota Bene (NB) [and beSocratic]

Review activities (what do you know already): gene-mutation-phenotype | BCI

Primary topic: Development in a single celled organism

 
nothing for the first day

but after class - questions to answer on Canvas to help me understand where you are at:

–What factors influence whether a protein is cytoplasmic or nuclear?
–What factors can influence whether a gene is expressed or not within a particular cell?
–How can one cell influence another?



 

Thursday,
17 January

Evolutionary mechanisms
+
Aggregative
multi
cellularity

 

 

 

 

 

Primary Topic: Evolutionary mechanisms and the origins of aggregative multicellularity

Activity: What is evolutionary theory meant to explain? outline (concept map) key topics in evolutionary processes

Steps in the evolution of multicellularity.

Q: What drives speciation (diversification) - multicellularity?
Q: Propose a plausible mechanism by which a point mutation could convert a social cooperator into a social cheater.
Q: What is social cheating? How does it differ from competition? How can it be suppressed?
Q: What is a public good? What is social cheaping?

 

read on NB before class: Multicellular behavior in bacteria: communication, cooperation, competition and cheating

read on NB before class: Boraas et al 1998. Phagotrophy by a flagellate selects for colonial prey: a possible origin for multicellularity. Evo. Eco. 12: 153-164.

background material: Oslaya et al. 2018. Cheating on cheaters stabilizes cooperation in Pseudomonas aeruginosa. Curr. Biol. 28: 2070-2080.

background material: Hillman et al. 2018. Multiple Roots of Fruiting Body Formation in Amoebozoa. Genome Biol. Evol.

 



 

Tuesday,
22 January

Clonal
multi-
cellularity
/
germ line
versus soma


Primary Topic: Evolutionary origins of (clonal) multicellularity (germ line versus soma)

Questions: What exactly are the important differences between mitotic and meiotic cell division?

What is the mechanism by which a cell determine whether all chromosomes are attached to a spindle

How do aggregative and clonal multicellularity differ.

 

read on NB before class: Brunet & King 2017. The origin of animal multicellularity and cell differentiation. Dev. Cell 43: 124-140.

optional watch: Nicole King : Origin of animal multicellularity (~12 minutes)



 

Thursday,
24 January

Inter-
cellular
signaling
basics

 

Primary Topic: Signaling basics origins of (clonal) multicellularity

Review: How can one cell influence another?
What do you know signaling and gene expression; how genes regulated?

how can mutations and other factors alter gene expression and function?

 

read on NB before class: Anja Rivera - A genetic toolkit.

background material: Watanabe et al., 2014/ Nodal signalling determines biradial asymmetry
in Hydra. Nature 515: 112.

background material: Babonis & Martindale. 2016. Phylogenetic evidence for the modular evolution of metazoan signalling pathways. Phil. Trans. R. Soc. B 372: 2015047



 

Tuesday,
29 January

Morphogen gradients

Primary Topic: Morphogen gradients  

read on NB before class: : Anja Rivera - Patterning

read on NB before class:



 

Thursday,
31 January

Embryonic
Axes
(left-right)

 

 

Primary Topic: Embryonic axes introduced - Left/Right

Q: What molecules are chiral at the cellular level?
Q: Outline steps in cell-cell signaling - potential effects of mutation, types of mutations.
Q: How can alleles interact physical versus genetic interactions
Q: Present a plausible model in which genetic background can influence an allele’s phenotype.

Core features of developing systems (asymmetry, induction, evolutionary adaptation)

 

read on NB before class: Lebreton et al., 2018 Molecular to organismal chirality is induced by the conserved myosin 1D. Science 362: 949-952

 

background material: Ray et al., 2018. Intrinsic cellular chirality regulates left-right symmetry breaking during cardiac looping. PNAS



 

Tuesday,
5 February

Embryonic
Axes
(A-P &
D-V)

 

 

Primary Topic: Establishing embryonic axes
Maternal asymmetries and zygotic inductions

 

Primary Topic: Morphogens, Signaling systems, and Turning processes.

 

 

 

read on NB before class: Genikhovish & Technau. 2017 On the evolution of bilaterality. Development.

background material:: Watanabe et al., 2014. Nodal signalling determines biradial asymmetry in Hydra. Nature.

Canvas: Design a plausible mechanism by which a gradient can be established in a single cell.
Does you model work of an ensemble of cells? Are modifications needed (described).



 

Thursday,
7 February

Model
Systems

Primary Topic: Selecting a Model Systems - strengths & limitations

 

  read on NB before class:  Perlman 2016. Mouse models of human disease: an evolutionary perspective. Evo. Med & Pub. Health.


 

Tuesday,
12 February

REVIEW

REVIEW - CATCH UP - review for exam    


 

Thursday,
14 February

MIDTERM #1

MIDTERM #1  First midterm exam    


 

Tuesday,
19 February

Xenopus: rotation, induction + differentiation

Primary Topic: What can we learn from the clawed frog Xenopus

oocyte maturation, cortical rotation, fertilization, MBT

 

read on NB before class:  Houston, 2010. Cortical rotation and messenger RNA localization in Xenopus axis formation.

read on NB before class: Briggs et al. 2018. The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution



 

Thursday,
21 February

Drosophila: mutations, patterning + the Hox system

Primary Topic: What can we learn from the fruit (vinegar) fly Drosophila   read on NB before class


 

Tuesday,
26 February

Nematodes:
lineages + specification

Primary Topic: What can we learn from the nematode C. elegans (lineage)   read on NB before class


 

Thursday,
28 February

Catch up

Primary Topic: Complete Drosophila and C. elegans   read on NB before class


 

Tuesday,
5 March

Chick (limb)

Primary Topic: What can we learn from the chick (limb formation)   read on NB before class


 

Thursday,
7 March

Neural crest


Primary Topic: What can we learn from the Neural crest
  read on NB before class


 

Tuesday,
12 March

Mammalian development

Primary Topic: Characteristic aspects of mammalian development    read on NB before class


 

Thursday,
14 March

Mouse-Human evoutionary differences

Primary Topic: Evolutionary (moelcular) differences between humans and other mammals. and human

  read on NB before class


 

Tuesday,
19 March

CATCH UP + Exam Review    


 
Thursday,
21 March
MIDTERM #2 Second midterm exam      


 
Spring Break      


 

Tuesday,
2 April

CNS patterning + neuronal pathfinding

Primary Topic: CNS patterning and Neuronal pathfinding

  read on NB before class


 

Thursday,
4 April

Stem cells

Primary Topic: Understanding stem cells part embryonic and hematopoetic   read on NB before class


 

Tuesday,
9 April

Stem cells

Primary Topic: Understanding stem cells: in the rest of the body.

  read on NB before class


 

Thursday,
11 April

ESC, iPSCs + organoids

Primary Topic: Embryonic stem cells : technical and moral questions Reprogramming somatic cells   read on NB before class


 

Tuesday,
16 April

human
organoids

Primary Topic: building tissues : iPSCs and organoids / Stem cells based therapies (an introduction)

  read on NB before class


 

Thursday,
18 April

epigenetic mechanisms

Primary Topic: Epigenetic mechanisms and development (fetal - maternal - paternal conflicts)   read on NB before class


 

Tuesday,
23 April

environmental effects on development

Primary Topic: Developmental defects ; fetal alcohol syndrome, thalidomide, etc.   read on NB before class


 

Thursday,
25 April

cancer development

Primary Topic: Developmental of cancer   read on NB before class


 

Tuesday,
30 April

open at
the mome
nt

open    


 

Thursday,
2 May

REVIEW

REVIEW for third midterm and comprehensive exam    


 

Monday,
7 May

FINAL
EXAM

FINAL EXAM + IKIN    

  Troll evolution animation
education

The original version of DEVO@CU by M.W. Klymkowsky/virtuallaboratory/beSocratic, llc.| part of the the biofundamentals cooperative
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. © 2019