Multiplicity of Infection 
Upon lysis, different cells release different numbers of phage. The question is why? One possibility is that the difference is due to the number of phage that infect each bacterium originally, e.g., a bacterium infected simultaneously by two phage might produce a larger (or a smaller) burst than bacteria infected by only one. The answer is that we don't
know if this is true, but we can design an experiment to find out. 
Lets do the experiment. We set up three bacterial cultures.
The phage absorb to the bacteria within a minute; now repeat the experiment as we did it last time. Dilute and aliquot the solution into 50 tubes, and determine the burst size for each MOI. We have automated this process. When you push the button, your vTA will determine the number of plates with phage, the number of phage per plate, the mean burst size and the standard deviation 


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revised 16 December 2004 